Regula Mühlemann was born in Adligenswil near Lucerne and studied at the Lucerne University of Applied Sciences and Arts with Prof. Barbara Locher. The young soprano gained her first experience on the opera stage at an early age at the Lucerne Theater. Subsequent engagements took her to the Teatro La Fenice in Venice as Despina (Così fan tutte), to the Zurich Opera House and to the Festspielhaus Baden-Baden, where she sang Giannetta in Donizetti's L'elisir d'amore.

Regula Mühlemann is an exclusive artist of Sony Classical and released her latest album Cleopatra - Baroque Arias (2017) after Mozart Arias (2016). 

In summer 2012, she made her debut as Young Papagena in the opera The Labyrinth by P. v. Winter at the Salzburg Festival. In the 2016/17 season, after a concert tour with the Verbier Festival Chamber Orchestra, Regula Mühlemann was heard in concerts at the Tonhalle Zurich, the KKL Lucerne and the Frauenkirche in Dresden, among others. She has also made her debut in the United States with the Chicago Symphony Orchestra under the baton of Manfred Honeck.

Other highlights of her career include Schumann's Das Paradies und die Peri with the Accademia Nazionale di Santa Cecilia under the musical direction of Daniele Gatti, as well as concert performances of Mozart's La clemenza di Tito at the Festspielhaus Baden-Baden in which Regula Mühlemann sang the role of Servilia alongside Joyce di Donato and Sonya Yoncheva. At the beginning of the 2015/16 season, Regula Mühlemann appeared in Mozart's Great Mass in C Minor and as Zerlina (Don Giovanni) in a concert performance alongside Erwin Schrott at the Yehudi Menuhin Festival Gstaad. This was followed by further concerts, including with the Basel Chamber Orchestra, the Chamber Orchestra of Europe conducted by Yannick Nézet-Séguin, the RIAS and Dresden Chamber Choirs, and a new staged production of Carl Orff's Carmina Burana at the Grand Théâtre de Genève. In addition, Regula Mühlemann has already made guest appearances at De Nederlandse Opera as Papagena in Mozart's Magic Flute, as Gretel (Hansel and Gretel) at the Teatro Regio in Turin, and has given numerous concerts, including at the Verbier Festival (Il Re pastore), at the Easter Festival in Baden-Baden and in Zermatt, where she appeared on stage with the Scharoun Ensemble.

Regula Mühlemann is also distinguished by her active concert career. She has performed many standard works of the classical concert literature from baroque to modern in Europe and in South America. She has worked with conductors such as Nello Santi, Simon Rattle, Daniel Harding, Enoch zu Guttenberg, Pablo Heras-Casado, Ivor Bolton, Ingo Metzmacher, Pinchas Steinberg, Gianandrea Noseda and Václav Luks. She is also a regular guest at the Lucerne Festival. Regula Mühlemann was a finalist in the Prix Credit Suisse Jeunes Solistes in Geneva and has received numerous prizes, including a scholarship from the Friedl Wald Foundation, the Migros Kulturprozent, the Elvira Lüthi Wegmann Foundation, the Armin Weltner and the Jmanuel and Evamaria Schenk Foundation. In 2015, she was a finalist in the Cardiff Singer of the World competition.